Basically, what you put in is what you get out, concentrated. As far as "seriously effect"? Depends on the quality of the trim and how much you mix it with your grade A material. Leaves do not contain much oil so adding leaves will effect yeild as well as add more lipds particularly if it is Outdoor, but if you are looking to be sustainable and utilize as much of your plant material as possible you may consider using the oil from C grade material for a separate product.